Prevalence and Antimicrobial Susceptibility Profiling of Salmonella in broiler farms at Patiya, Chittagong A Clinical report Submitted as per approved style and contents Faculty of

Abstract

The aim of this study was to determine the prevalence of Salmonella infections in broiler farms and their antimicrobial susceptibility profiling test at Patiya, Chittagong. Bacteria isolated from pooled fecal samples of broiler at Patiya, Chittagong were screened for the presence of Salmonella spp. A total of 30 pooled fecal samples were tested during 01 March 2017 to 06 April 2017 and 05 July 2017 to 03 August 2017 at PRTC (Poultry Research and Training Centre), CVASU (Chittagong Veterinary and Animal Sciences University), where 9 (30%) samples were positive for Salmonella through all classical bacteriological tests. Isolated Salmonella were tested for resistance to 5 different antimicrobial agents such as Amoxicillin, Ampicillin, Ciprofloxacin, Salfamethazole and Colistin sulphate which was carried out by the Kirby-Bauer disc diffusion method as per recommendation of CLSI and efficacy of antibiotics was determined by measuring the diameter of the zone of inhibition that results from diffusion of the agent into the medium surrounding the disc. The Salmonella were found 88.89%, 88.89% and 66.67% resistance to Amoxicillin, Ampicillin and Salfamethazole respectively. 77.78% samples were sensitive & 11.11% samples were intermediate to Colistin Sulphate which was the highest in sensitivity followed by 55.56% sensitive to Ciprofloxacin. The growth of resistance against antibiotic was due to carelessly using antibiotic through farm owner without maintaining dosage prescribed by veterinarians.