IN-SILICO EVALUATION OF BIOACTIVITY OF PHYCOCYANIN FROM RED ALGAE

Abstract

Phycocyanin (PC) is a neutraceutical compound with biological action which is extracted and purified from seaweeds. It is found in red algae, blue-green algae and different seaweeds. According to the Algae Base dynamic species count, there are roughly 10,000 species of seaweeds, the vast majority of which are red algae. The percentage of protein in dried red seaweed varies between 20 and 47 percent (dw), so it’s proteins are potential precursors for showing bioactivity. In this present study, seventeen proteins of red algae (Pyropia endiviifolia, Pyropia pulchra, Neopyropia yezoensis, Neoporphyra haitanensis, Pyropia fucicola, Pyropia kanakaensis, Neoporphyra dentata, Neoporphyra dentata, Galdieria sulphuraria, Polysiphonia urceolata) have been selected as potential precursors of bioactivity based on in silico approach. In silico analysis of Phycocyanin performed high numbers of peptides angiotensin-I-converting enzyme (ACE-I), dipeptidyl peptidase-IV (DPP-IV) and Alpha glucose inhibitor. Chymotrypsin, papain, thermolysin and stem bromelain have been used in-silico proteolysis. For that reason, 45 different tripeptides and dipeptides are tested to see whether any of them can be considered novel bioactive peptides. The distinctive features of the peptides have been explored using Peptide Ranker, PepCalc, Peptide Cutter, ToxinPred, AllerTop, and AHTpin. Bioinformatics analysis indicates that the vast majority of the peptides are likely to be non-toxic, very promising, and safe for use. Future in-vitro and in-vivo studies of the bioactivity of phycocyanin from red algae can be based on these results. This research emphasizes the promise of phycocyanin from red algae as a base material for the creation of novel meals and medicines. Keywords: Red algae, Phycocyanin, Bioactive peptide, In silico, ACE inhibitor, DPP IV inhibitor, Alpha Glucose inhibitor.