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|MOLECULAR CHARACTERIZATION OF LEPTOSPIRA HARDJO IN CATTLE
|A thesis submitted in the partial fulfillment of the requirements for the degree of Master of Science in Medicine Department of Medicine and Surgery Faculty of Veterinary Medicine Chittagong Veterinary and Animal Sciences University Chittagong-4225, Bangladesh
|Leptospirosis is a potential threat to dairy industry responsible for early embryonic death and infertility which can lead to significant economic losses for cattle producers. A cross sectional study was conducted for molecular characterization of Leptospira Hardjo in sero-positive dairy cows by using polymerase chain reaction (PCR) technique. Organisms were isolated from urine and fetus by using Ellinghausen-McCullough-Johnson-Harris (EMJH) media and initial tracing by dark field microscopy from 45 sero-positive selected cows. Prevalence of Leptospira Hardjo in dark field microscopy was 55.55% (25 out of 45) and 32% (8 out of 25) respectively in urine and fetus samples. No band was found in PCR from urine samples (none out of 45) besides 32% (8 of 25) fetal samples were found positive for L Hardjo. Maximum cases of abortion were occurred during the second trimester of pregnancy and their difference among the gestation varies significantly (P<0.05). The overall results of this study expressed that Leptospira Hardjo is one of the potential causes of abortion in the dairy industry of Chittagong, Bangladesh.
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