ANALYSIS OF BIOACTIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY OF RARE FRUITS OF BANGLADESH

Abstract

Antioxidant activity and bioactive compounds are the major quality attributes of fruits owing to the evidence of many epidemiological studies from many parts of the world for reducing the risk of chronic degenerative diseases. Studies indicate that rare fruits are the rich sources of polyphenol, flavonoids, anthocyanin and antioxidants. The objectives of this studies are to determine the concentration of antioxidants, three bioactive compounds, analyze significant correlation. Antioxidant activity (AOA) was undertaken by DPPH(2,2-Diphenyl-hydrazyl-hydrate) assay. High activity being found in Katajamin (303 mg/100 g) and low activity observed in Pani Fol (55mg/100 g) and average was 130 mg/100 g of extract. The bioactive compounds are total polyphenol content (TPC) of the extract by the Folin-Ciocalteu method ranged from Ashari (10 ±2.19) to Katajamin (41±34.98) mg GAE/g (gallic acid equivalent) and average was 60 mg GAE/g. Total flavonoid content (TFC) was varied from Ashari (06±3.94 mg QE/g) (Quercetin equivalent) to Katajamin (76±6.67 mg QE/g) with average 42 mg QE/g using aluminum chloride colorimetric method. Again, anthocyanin most significant coloring pigments in fruits was highest in Bet Fol (102±3.46 mg TA/100 g) and minimum was in Ashari (02±0.74 mg TA/100 g). There was a positive correlation between antioxidant activity and bioactive compounds where a significant (P≤0.05) correlation observed between AOC with TPC and total TFC. Again a strong positive correlations were find among three bioactive compounds where TPC and TFC were significant (P≤0.05) but other two like TPC Vs TAC and TFC Vs TAC were not significant (P≥0.05). So, important messages from this study are, majority of the rare fruits are the important sources of antioxidant and bioactive compounds and these fruits can be used in food processing industry for nutraceutical product development.