Please use this identifier to cite or link to this item: http://dspace.cvasu.ac.bd/jspui/handle/123456789/842
Title: DEVELOPMENT OF PROBIOTIC FRUIT JUICE USING LACTIC ACID FORMING BACTERIA ISOLATED FROM ‘DAHI’ WITH SUPPLEMENTATION OF PREBIOTICS
Authors: Akter, Fahmida
Keywords: Lactic Acid Bacteria, Polymerase Chain Reaction (PCR), Probiotic Orange juice, Prebiotic.
Issue Date: Dec-2019
Publisher: Chattogram Veterinary & Animal Sciences University
Abstract: Usually probiotics are consumed in dairy based product such as dahi or yogurt. Dahi is a widely consumed fermented dairy product in Bangladesh. However, given the rise in various diet types, non-dairy alternatives have been developed such as inoculating fruit juices with probiotics. This study was carried out to develop a probiotic fruit juice using lactis isolates from dahi with prebiotic fortification. This study indicated an alternate effective way for those who are allergic or intolerant to dairy based products. Dahi samples were collected from different local shops of Chattogram and Bogura, Bangladesh. Lactic acid forming bacteria were isolated from the samples by using MRS (deMan, Rogosa and Sharpe) media. The presumptive isolates were identified on the basis of their morphological and biochemical characteristics. Gram’s staining and Catalase test were done in this regard. Furthermore, these isolates were confirmed by Polymerase Chain Reaction (PCR) using specific primers sets designed for conserved 16S rDNA regions of Lactobacillus genus and Lactococcus lactis sp. Lactis isolates were confirmed and further used for the development of probiotic fruit juice. Prebiotic (asparagus & ginger powdered form) was added in fruit juice to support the growth and development of inoculates. Streaking plate technique was performed to check the growth of inoculated isolates. Growth of the bacterial colony on petri agar medium showed that asparagus exerted a positive effect on the survival of lactic acid bacteria in orange fruit juice.
URI: http://dspace.cvasu.ac.bd/jspui/handle/123456789/842
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