Please use this identifier to cite or link to this item: http://dspace.cvasu.ac.bd/jspui/handle/123456789/392
Title: NUTRACEUTICAL STUDY AND POTENTIAL ANTIDIABETIC ACTIVITY (in-vitro) OF TWO SPECIES OF TROPICAL WATER LILY
Other Titles: (Nymphaea nouchali & Nymphaea rubra)
Authors: Nishan, Kanij Fatema
Keywords: Nymphaea, Bioactive, Antioxidant, DPPH, α-amylase.
Issue Date: Dec-2020
Publisher: Chattogram Veterinary and Animal Sciences University Chattogram-4225, Bangladesh
Abstract: Plant extracts, different parts of plant even the whole plants of water lily act as a source of remedies are widely used for the prevention or treatment of many diseases apart from their nutritional value. The present work was aimed to evaluate the nutraceutical value of three parts (Petal- PS1, PS2; Seed- SS1, SS2; Tuber- TS1, TS2) of Nymphaea nouchali and Nymphaea rubra respectively by means of assessing nutritional composition (Moisture, Ash, crude fat, crude fiber, crude protein, CHO and vitamin C), mineral content (Na, K, Mg, Ca, P, Fe, Cu, Zn), bioactive phytochemical content (Total phenol, total flavonoid, tannin, saponin, alkaloids) using standard method. In vitro DPPH assay and α-amylase inhibition assay were conducted to identify their potential antioxidant and antidiabetic activity respectively. The result show that tuber sample (TS1, TS2) had higher moisture and ash content, while seed sample (SS1, SS2) had higher crude fat and carbohydrate content. Petal sample (PS1, PS2) had higher crude protein and crude fiber content. Vitamin C content is higher in tuber sample than other water lily parts. Macro and micro minerals (K, Ca, P, Fe, Cu and Zn) were higher in petal sample, Na and Mg were higher in seed (SS1) and tuber (TS1) respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids. Total phenolic content (5.597±0.01 mg GAE/100g) and flavonoid content (794.315±0.86 mg QE /100gm) were found to be higher in petal sample (PS1) of N. nouchali than N. rubra. Lower IC50 value found in petal sample (PS1: 6.91 μg/mL and PS2: 12.70 μg/mL) indicate that ethanolic extracts of petal sample possess higher antioxidant activity that can scavenge more DPPH free radicals. Nymphaea nouchali and Nymphaea rubra inhibited the activity of α-amylase with an IC50 value of 13.25 μg/mL and 27.21 μg/mL in petal; 60.69 μg/mL and 55.24 μg/mL in seed; 59.71 μg/mL and 50.89 μg/mL in tuber respectively. Whereas, the positive control Acarbose used in this study, shows an IC50 value of 22.52 μg/mL. Nutritional composition obtained in this study suggest that petal, seed and tuber of both N. nouchali and N. rubra have potential food value and could be recommended as a functional food ingredients. They are also a promising source of potential antioxidants and may be efficient for the prevention of diabetes. However, the strength of existing data is not enough. Further in vivo studies will be needed to determine the main active ingredient behind its efficiency.
URI: http://dspace.cvasu.ac.bd/jspui/handle/123456789/392
Appears in Collections:Thesis-MS

Files in This Item:
File Description SizeFormat 
Nishan Thesis (black binding).pdf2.09 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.