PROTEOMICS OF THE RED ALGAE Pyropia umbilicalis (PYROPIA, RHODOPHYTA)

Abstract

There has been only a little expansion of proteomics' use in the study of algae. This study details the process of determining the proteome of the economically significant red seaweed Pyropia umbilicalis. For determination of the molecular properties of the collected red seaweed sample a partial DNA test was carried out, that was analyzed in NCBI BLAST for sequence homology. A protein extraction method with a combination of treatment using cellulase enzyme, sonication and ammonium sulphate extraction was employed to extract Pyropia umbilicalis proteins. Extracted protein was digested using trypsin in-solution digestion, and air drying was employed to reduce the volume of digested sample. LC-MS analysis was used to identify the functional proteins which are as follows R-phycoerythrin beta chain, R-phycoerythrin alpha chain, B-phycoerythrin beta chain, C-phycocyanin alpha chain, C-phycocyanin beta chain, Ribulose bisphosphate carboxylase large chain, Allophycocyanin beta chain, and Allophycocyanin alpha chain. Total yield of extracted protein was 33.494±0.709066 %. and concentration of total extracted soluble protein was 0.75±0.002% following the modified version of the Bradford assay. These findings emphasize the potential of employing proteomic techniques to investigate the Pyropia umbilicalis protein function.