IN SILICO APPROACH TO CHECK THE EFFECT OF RED ALGAE Pyropia umbilicalis

Abstract

Pyropia umbilicalis is an important marine algae. Its high protein concentration makes it a good source of peptides that are physiologically active. A time saving and effective way for determining the potential bioactivities of any protein is made possible by the growing field of bioinformatics and the numerous databases of bioactive peptides.The current in-silico study demonstrate the biotechnological characteristics of P. umbilicalis by identifying bioactive peptides within its protein. In this study,ficin, papain, and stem bromelain, three plant proteases, were utilized to in silico proteolyse five different P. umbilicalis protein sequences and release a variety of bioactive peptides. The BIOPEP-UWM database was used to assess the efficacy of these enzymes. Stem bromelain was detected to be more efficient at releasing fragments for a given activity. From these proteins, 22 biological activities were identified in total. The findings demonstrated that P. umbilicalis protein is a possible source of peptides with dipeptidyl peptidase-IV (DPP-IV) and angiotensin-I converting enzyme (ACE) inhibiting properties. The protein sequence of P. umbilicalis was compared to species possessing the same protein using NCBI database.It was found that protein sequence of Palmaria palmate,an economically beneficial seaweed has similarity (positivity, 92%– 95%) with this seaweed protein sequence. The results of the current study suggest that P. umbilicalis protein can be a great source of biologically active peptides, and these results pave the way for novel applications of peptides in pharmaceuticals, biomedicine, and the food industry. Future research on the bioactive peptides generated from these seaweed proteins can be supported with these findings in both in vitro and in vivo settings.