ASSESSMENT OF TOTAL PHENOLICS, TOTAL FLAVONOIDS CONTENT AND ANTIOXIDANT CAPACITIES AND PHYTOCHEMICALS SCREENING OF SOME SELECTED HERBS

Abstract

The research study was conducted to comprehend the bioactivity and phytochemical screening of Coriandrum sativum( Dhaniya), mentha spicata(Tulshi), ocimum sanctum( pudina) and centella asiatica (Thankuni) using methanol and ethanol as solvent for extraction. Phytochemicals were examined by the standard test methods for different chemical group found in plants. The preliminary phytochemical analyses showed that the extracts contain Alkaloids, Flavanoids, Tannins, Saponin and Carbohydrates. Through using Folin–Ciocalteu assay the Total phenolics were measured and based on aluminium chloride method total flavonoids content estimation was carried out in the sample extract by spectrophotometrically. For the estimation fo the total antioxidant capacity, the DPPH (1,1-diphenyl-2-picrylhydrazyl radical) method was introduced. To find out the level of significance at P <0.05,one way analysis of variance was performed. Total phenolic content of ethanolic extract of the plant was ranging from 72.83±0.001 to 34.37±0.005 mg gallic acid equivalents/ g dry weight. However, estimated phenolic content in methanolic extract ranges from 69.74±0.001 to 21.502±0.05 mg gallic acid equivalents/g dry weight. These findings reveal the ethanolic extract contains more potential antioxidant capacity. The leaves extract in ethanolic solvent was highly enriched with both phenolic and flavonoid contents compared to methanolic extract. A significant positive correlation was observed between TPC determined using Folin-Ciocalteu and the DPPH (1,1-diphenyl-2-picrylhydrazyl radical) method. The outcome suggests that phenolic chemicals are the main driver of these medicinal plants' antioxidant action. From the results, it is to conclude that these plants might be a reliable source of free radicals for food applications.