Identifying Phytochemical Constituents of Coccinia grandis and Identifying Efficacy of Coccinia grandis Against Salmonella spp and E coli

Abstract

Medicinal plants are a source of great economic value. Coccinia grandis or Telakucha is a famous medicinal plant which is growing all over the world.The Present study was conducted to evaluate the phytochemical screening of ethanolic and methanolic extract of Coccinia grandis and the effect of ethanolic extract against Salmonella spp, and E. coli from Poultry sample (September 2021 to February 2022) at Chittagong Veterinary Animal Science University (CVASU), Bangladesh. Telakucha leaves were collected from different area of Kelishahar under the upazilla patiya which is situated in Chattogram. Then leaves of Telakucha were then immersed in Ethanolic and Methanolic extracts for seven days, Telakucha leaves were conducted phytochemical screening for proving the presence of Alkaloids, Flavonoids, Saponins, Tannins, Phenolic Compound, Glycosides, Carbohydrates, Reducing sugar, Protein and Amino acid, Acidic Compound, Phytosterol and finally Steroids and Terpenes. In the present study, a systematic approach was followed to develop and standardize the process. Phytochemical screnning of the extract of telakucha was done by different test and antimicrobial succeptibility test for E. coli and Salmonella spp. was occurred by Agar Disc Diffusion Method. These bacteria grew in Mueller Hinton agar and observed the sensitivity after 24 hours.In this study the phytochemical component like alkaloids, flavonoid, saponins, phenols, tannins, terpenoids etc are present in Telakucha.This phytochemical constituents contain antimicrobial efficacy that inhibit the growth of two gram negative bacteria such E. coli and Salmonella spp . All concentration showed positive results after successfully applying the extract of Telakucha in the petri dish. At 40% concentration showed the highest zone of inhibition than other the two concentrations.