DETERMINATION OF MATERNAL ANTIBODY AGAINST NEWCASTLE DISEASE, TRANSFER FROM HEN TO CHICKS BASED ON HI TEST

Abstract

A study was conducted to asses the antibody titer against Newcastle disease virus in the breeder flock and the amount of antibody transfer from hen to egg and day old chick (DOC). The study was commenced from 6th September 2008 to 8th October 2008. Blood samples were collected from the Broiler parent stock reared in environmental control system. Twenty blood samples were collected from each of two flock where 5000 birds in each flock. Twenty eggs samples were collected from each flock in the same day. The day old chicks were collected 21 days after the date of incubation from the same lot. After collection of serum from the blood of hen and day old chicks, yolk from eggs were analyzed in Microbiology laboratory, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong. The antibody titer was analyzed based on HI test. For New castle disease virus antigen used New castle disease virus vaccine. The result shows that 42 weeks old hen from Flock 1 possess antibody titer (Log28.15±0.37). The antibody titer of the collected eggs of same flock was (Log25.8±0.20).The antibody titer in chick was (Log26.15±0.28). The level of antibody titer represents a transfer rate from hen to egg (71.17%). On the other hand, the transfer rate of antibody titer from hen to day old chick shows more promising result, at a transfer rate (74.85%).The antibody titer of Flock 2 shows somewhat different result than that of flock 1. Here the titer in the laying hen of 65 wks age was (Log28.45±0.34) while in egg yolk contained (Log26.1±0.289) and chicken serum had (Log26.1±0.289). This result shows that the transfer rate from hen to eggs was (72.19%) and hen to day old chicks was (72.19%).So, there is a relation of antibody titer between hens, their eggs and also their chicks. By examining the antibody titer of eggs, we can say the approximate antibody titer of hen and the chicks of same lot.