DSpace Collection:http://dspace.cvasu.ac.bd/jspui/handle/123456789/192024-03-29T07:53:47Z2024-03-29T07:53:47ZMODIFIED PLASTINATION TECHNIQUE FOR TEACHING AND RESEARCHNOVA, FARNAZ KADERhttp://dspace.cvasu.ac.bd/jspui/handle/123456789/20112023-08-20T10:50:43Z2022-06-01T00:00:00ZTitle: MODIFIED PLASTINATION TECHNIQUE FOR TEACHING AND RESEARCH
Authors: NOVA, FARNAZ KADER
Abstract: Plastination is recognized as one of the most proficient tissue preservation procedures that has a wide range of applications in education and research. This technique demands costly equipment and chemicals; which requires adapting this exquisite process in an affordable manner. This study intends to develop an affordable methodology for preparing luminal casts and sheet plastinates with commercial silicon sealant following the basic steps (fixation, dehydration, impregnation and curing) of traditional plastination procedures. The resulting luminal castings were environment-friendly, dry, odorless, flexible, non-irritant and provided three-dimensional representations of the bronchial tree, segmentation of renal artery and orientation of the external auditory canal of goat. On the other hand, sheet plastinates were semi-transparent, dry, portable and presented good anatomical details of brain, spinal cord and kidney sections. Students of the Gross Anatomy course were approached about the use of plastinated and formalin-preserved specimens through a structured questionnaire. Almost all of them (95.5%) felt unsafe during handling of formalin-fixed specimens, 91.1% of students regarded plastinated samples very safe during hand manipulation and none reported any odor or health threat from the plastinates. Furthermore, 51.1% of participants strongly suggested the plastinates in anatomy practical courses, while 48.9% recommended the plastinates in conjunction with fixed and fresh samples and 88.9% found the plastination technique very essential for studying anatomy. Finally, the modified procedure may be utilized for anatomy instruction and research at a lower cost than the traditional technique. However, this approach is time-consuming, requires expert staff and cannot substitute conventional hands-on dissection for comprehensive anatomical knowledge.2022-06-01T00:00:00ZPOSTNATAL GROWTH AND DEVELOPMENT OF LYMPHOID ORGANS AND TISSUES IN DESHI CHICKEN (GALLUS DOMESTICUS) OF BANGLADESHHasan Sohel, DR. Md. Shahriarhttp://dspace.cvasu.ac.bd/jspui/handle/123456789/19212023-06-19T05:51:14Z2018-06-01T00:00:00ZTitle: POSTNATAL GROWTH AND DEVELOPMENT OF LYMPHOID ORGANS AND TISSUES IN DESHI CHICKEN (GALLUS DOMESTICUS) OF BANGLADESH
Authors: Hasan Sohel, DR. Md. Shahriar
Abstract: The gross and histomorphometrical studies were conducted in scavenging and captive deshi chickens of Bangladesh to know the comparative postnatal growth and development of lymphoid organs and tissues during the period of March to December, 2017. Total 40 non-descriptive deshi chickens were reared in scavenging and intensive system (20 chickens for each) from day 1 to 180 according to standard rearing system. Gross examinations of different lymphoid organs were performed for different ages (day 1, day 30, day 90 and day 180) of both types of rearing chickens. For histomorphometrical study the tissue samples were fixed, processed and stained with H & E stain according to the standard histological procedure and AmScope image measurement software were used for histomorphometry. The gross weight, length and width of thymus, spleen and cecal tonsils were gradually increased significantly (P≤0.05) with the advancement of ages in both scavenging deshi chickens (SDC) and captive deshi chickens (CDC) but the weight, height and width of bursa were decreased at D180 in both types of reared chickens. In both SDC and CDC, the highest weight (1.88 ± 0.13 and 2.04 ± 0.11 gm, respectively), height (1.26 ± 0.11 and 1.48 ± 0.08 cm, respectively) and width (0.76 ± 0.09 and 1.04 ± 0.11 cm, respectively) of bursa was found at D90 but the highest number of thymic lobe (6.40 ± 0.54 and 6.40 ± 0.54, respectively), weight (0.98 ± 0.08 and 1.26 ± 0.11 gm, respectively) and length (3.68 ± 0.08 and 3.78 ± 0.08 cm, respectively) of thymus; the highest weight (2.78 ± 0.16 and 3.06 ± 0.18 gm, respectively), length (1.98 ± 0.08 and 2.26 ± 0.16 cm, respectively) and width (1.42 ± 0.08 and 1.62 ± 0.08 cm, respectively) of spleen and the highest weight (2.60 ± 0.10 and 2.72 ± 0.08 gm, respectively), length (1.70 ± 0.10 and 1.82 ± 0.08 cm, respectively) and width (1.18 ± 0.08 and 1.30 ± 0.10 cm, respectively) of cecal tonsil was found at D180. All the gross parameters were found significantly (P≤0.05) higher in CDC as compared with SDC. Similarly, the histomorphometrical parameters such as length and width of thymic lobules, white pulps of spleen and lymphatic nodules of cecal tonsils were also increased significantly (P≤0.05) with the advancement of ages in both SDC and CDC but height of epithelium, number and height of mucosal folds, number of follicles per mucosal fold of bursa were decreased at D180 in both SDC and CDC. All those histomorphometrical parameters were found significantly (P≤0.05) higher in CDC as compared with SDC. So, it is concluded that the higher gross and histomorphometrical parameters of lymphoid organs of captive deshi chickens than that of scavenging deshi chickens were due to their different rearing system.2018-06-01T00:00:00ZRELIABLE DIAGNOSTIC TECHNIQUES FOR CANINE DIABETES WITH HISTOPATHOLOGICAL EFFECTS OF ORGANSPoddar, Sonnethttp://dspace.cvasu.ac.bd/jspui/handle/123456789/19202023-06-19T05:47:11Z2018-09-01T00:00:00ZTitle: RELIABLE DIAGNOSTIC TECHNIQUES FOR CANINE DIABETES WITH HISTOPATHOLOGICAL EFFECTS OF ORGANS
Authors: Poddar, Sonnet
Abstract: The study was planned to find out a reliable diagnostic techniques for canine diabetes with
histopathological effects on different organs. A total number of 53 candidate diabetic dogs
from SAQTVH, CVASU and Chittagong metropolitan area were studied. Diabetes in dogs
were determined on the basis of clinical finding, hematological profile and serum
biochemical profile evaluation. Organs specimens from a dead diabetic and healthy dog were
collected for histopathological studies. The prevalence of diabetic dog was 5.76%. The mean
blood glucose parameter in diabetic dog was 11.5±0.42 mmol/L. TLC (10.48±0.32 ×103 /μl),
ALT (143.38±2.66 IU/L), cholesterol (237.68±0.43 mg/dl) and glycated haemoglobin
(9.51±0.31%) values were significantly elevated in diabetic dogs than that of control dog.
Glycated haemoglobin (HbA1c %) test was more reliable than portable blood glucose
measurement device test, oral glucose tolerance test and hematological profile evaluation test.
Histopathologically, the number and size of pancreatic islet were reduced with degeneration,
fatty deposition, fibrosis and necrosis. The villus length of intestinal mucosa was shorter (1.1
mm) in diabetic dog than healthy dog (1.5 mm). Cellular infiltration and fatty deposition were
observed in liver of diabetic dog. HbA1c test might be useful to clinician for effective
diagnosis of canine diabetes and histopathology of different organs might provide knowledge
about deleterious effects on organs.2018-09-01T00:00:00Z